Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

Article Title: DUSP1 protein's impact on breast cancer: Anticancer response and sensitivity to cisplatin.

doi: 10.1016/j.bbagrm.2025.195103

Figure Lengend Snippet: Fig. 1. Reduction of DUSP1 expression with siRNAand decreased cell proliferation. The expression of DUSP1 was attenuated by transfection of DUSP1-siRNA into MDA-MB-231, MDA-MB-468 and BT20 cells. Western blot analysis (A, B, and C) revealed a significant decrease in DUSP1 levels. Cell proliferation was monitored immediately after seeding, and the cell count at 24 h served as the baseline for further assessment. The fold-change in proliferation (D, E, F) demonstrates a notable decrease following DUSP1 suppression in both cell lines. Data are represented as the mean ± standard error of the mean (SEM); n = 4; * p < 0.05, versus Control-siRNA.

Article Snippet: For each well, 4 μl of siRNA transfection reagent (Qiagen, #301705), 100 μl of transfection medium, 1 μg of control siRNA (Sc-3707, Santa Cruz, USA), and human DUSP1 siRNA (Sc-35,937, Santa Cruz, USA) were used.

Techniques: Expressing, Transfection, Western Blot, Cell Counting, Control

Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

Article Title: DUSP1 protein's impact on breast cancer: Anticancer response and sensitivity to cisplatin.

doi: 10.1016/j.bbagrm.2025.195103

Figure Lengend Snippet: Fig. 10. DUSP1 overexpression enhances cell proliferation and reduces p38 phosphorylation. DUSP1 expression was increased by transfecting MDA-MB 231 and MDA-MB 468 cells with cDUSP1. Western blot analysis (B, D) confirmed a marked elevation in DUSP1 protein levels 72 h post-transfection. Cell proliferation was continuously monitored, with the cell index at 24 h used as the normalization baseline. The normalized proliferation index (A) indicated a significant increase in proliferation following DUSP1 overexpression. Concurrently, a decrease in phosphorylated p38 levels was observed, while ERK1/2 phosphorylation remained unchanged (B). In contrast, pharmacological inhibition of DUSP1 with BCI (50 nM, 24 h) led to an increase in phosphorylated p38 without affecting ERK1/2 phosphorylation (C). Data are presented as mean ± standard error of the mean (SEM), n = 4; *p < 0.05 versus Control-cDNA.

Article Snippet: For each well, 4 μl of siRNA transfection reagent (Qiagen, #301705), 100 μl of transfection medium, 1 μg of control siRNA (Sc-3707, Santa Cruz, USA), and human DUSP1 siRNA (Sc-35,937, Santa Cruz, USA) were used.

Techniques: Over Expression, Phospho-proteomics, Expressing, Western Blot, Transfection, Inhibition, Control

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet:

Article Snippet: Subcutaneous Preadipocyte Differentiation Medium , ZenBio , Cat#DM-2.

Techniques: Infection, Recombinant, Electron Microscopy, SYBR Green Assay, Lysis, Protease Inhibitor, Plasmid Preparation, XF Assay, Isolation, Quantitation Assay, Bicinchoninic Acid Protein Assay, Software